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Mechanistic and Characteristic investigation of recombinant eukaryotic CD151 and prokaryotic HpIMPDH proteins

October 25, 2018 @ 11:00 am - 12:00 pm

Expression of recombinant proteins has been carried out for many years in the number of host systems like bacteria, yeast, plants, transgenic animals and both cultured insects and mammalian cells. Compared to other systems, bacterial system (E.coli) remains the best candidate expression system since they are highly cost-effective, well-studied genetics, high yield and short doubling time. The application of
recombinant protein production is widely present in different aspect of proteomics especially in functional
characterization, biochemical studies and structural biology that requires pure and high yield of the protein. Here, we have studied heterologous expression (in E.coli) followed by functional & structural characterization of eukaryotic (Homo sapiens) transmembrane protein tetraspanin CD151 and prokaryotic (Helicobacter pylori) Inosine-5’-monophosphate dehydrogenase. CD151 is considered the most challenging proteins to express in the bacterial heterologous system, and the HpIMPDH is another equally difficult protein to express in soluble and active form.Discipline/Coordinating Entity: Biological Engineering


October 25, 2018
11:00 am - 12:00 pm
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